Journal: STAR Protocols

Article Title: In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids

doi: 10.1016/j.xpro.2022.101751

Figure Lengend Snippet: 3D cell death analyses of confocal images of an I/R CS using Imaris software 3D rendering analyses of confocal images of an I/R CS stained with ethidium homodimer (yellow), and with antibodies against CD31 for endothelial cells (blue), cTNT for cardiomyocytes (red) and vimentim for fibroblasts (green). (A–D) (A) shows overlay of (B) highlighting all the dead cardiomyocytes, (C) highlighting dead endothelial cells and (D) highlighting dead fibroblasts, respectively. Scale Bar: 100 μM. Reused from ( Sharma et al., 2022 ).

Article Snippet: Figure 3 3D cell death analyses of confocal images of an I/R CS using Imaris software 3D rendering analyses of confocal images of an I/R CS stained with ethidium homodimer (yellow), and with antibodies against CD31 for endothelial cells (blue), cTNT for cardiomyocytes (red) and vimentim for fibroblasts (green). (A–D) (A) shows overlay of (B) highlighting all the dead cardiomyocytes, (C) highlighting dead endothelial cells and (D) highlighting dead fibroblasts, respectively.

Techniques: Software, Staining

Journal: STAR Protocols

Article Title: In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids

doi: 10.1016/j.xpro.2022.101751

Figure Lengend Snippet:

Article Snippet: Figure 3 3D cell death analyses of confocal images of an I/R CS using Imaris software 3D rendering analyses of confocal images of an I/R CS stained with ethidium homodimer (yellow), and with antibodies against CD31 for endothelial cells (blue), cTNT for cardiomyocytes (red) and vimentim for fibroblasts (green). (A–D) (A) shows overlay of (B) highlighting all the dead cardiomyocytes, (C) highlighting dead endothelial cells and (D) highlighting dead fibroblasts, respectively.

Techniques: Isolation, Recombinant, Saline, Plasmid Preparation, Software, Microscopy, Imaging

Journal: Biomedical Optics Express

Article Title: Brain virtual histology with X-ray phase-contrast tomography Part II:3D morphologies of amyloid- β plaques in Alzheimer’s disease models

doi: 10.1364/BOE.438890

Figure Lengend Snippet: Representative 2D overlay and 3D orthographic rendering (AMIRA) of segmented plaques for each strain.

Article Snippet: This strategy proved versatile and accommodated the different types and numbers of plaques imaged in this study ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 4. caption a7 Representative 2D overlay and 3D orthographic rendering (AMIRA) of segmented plaques for each strain.

Techniques:

Journal: JACC Case Reports

Article Title: Stabbed Through the Heart

doi: 10.1016/j.jaccas.2019.12.048

Figure Lengend Snippet: Intraprocedural Transesophageal Echocardiogram Intraprocedural transesophageal echocardiogram (TEE) from a modified 4-chamber view showing the Amplatzer delivery catheter across the ventricular septal defect (top left) ; 3D echocardiography showing deployment of the Amplatzer VSD Occluder (top right) ; TEE showing the Amplatzer occluder device post-deployment (bottom left) ; 3D TEE with transillumination showing the post-procedure Amplatzer device from the perspective of the RV (bottom right) . Abbreviations as in Figures 1 and .

Article Snippet: Transillumination is a new 3D rendering tool (Philips, Amsterdam, the Netherlands) that uses a freely movable virtual light to enhance image details and depth perception; this tool was useful in the visualization of our patient’s VSD ( , ) ( ).

Techniques: Modification

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: (A) A 3D-printed off-the-shelf acetabular cup. An area of the porous layer has been enlarged, displaying its structure. (i) Pore size and (ii) strut thickness are indicated. The diameter of the pore is calculated as the diameter of a circle of equal area to the shape indicated in red. (B) A mesh structure from a 3D-printed cup rendered in analysis software (Simpleware, Synopsys, Exeter, UK), where the colours indicate variability in strut thickness in the porous layer.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Pore Size, Software

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: A panel of SEM images depicts surface-adhered particles (indicated) in the porous layer of 3D-printed acetabular cups, and the variability of the particles with 3D-printing methods; electron beam melting (EBM) and selective laser melting (SLM). All images at ×200 magnification.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques:

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: A 3D-printed implant (e.g. (A) an acetabular cup). A micro-CT scan of the implant can assist in determining (B) the diameter of the implant and provide (C) isolated slices of the internal structure for measurement of (D) the thickness of the porous region, (E) the dense region, and (F) the total thickness.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Micro-CT, Isolation

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: (A) Image generated from micro-CT data to show voids in the dense region of a 3D-printed acetabular cup. From these images, void location and frequency can be analysed. This will be followed by (B) void size and shape evaluation.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Generated, Micro-CT

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: A selection of 3D-printed custom and off-the-shelf acetabular cups, from a range of manufacturers and 3D-printing methods. Certain features of the cups can start to be considered, such as the locations of the porous regions and the different types of porous structures.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Selection

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: A summary of the features of a 3D-printed acetabular cup.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Pore Size

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: (A) SEM images of a regular or cellular and an irregular 3D-printed porous structure and the corresponding manufacture method (electron beam melting (EBM) and selective laser melting (SLM)). (B) Computer-rendered meshes formed using micro-CT data from 3D-printed acetabular cups from four separate manufacturers with varying porous structures. Comparisons between porous structures available from different manufacturers can be made.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Micro-CT

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK). From this data, (B) the porous layer is examined and isolated, and (C) a mesh model of the structure is generated. (D) A single mesh unit can then be extracted, followed by (E) best fit modelling with a sphere, to assist in calculating the porosity of the porous layer.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Micro-CT, Software, Isolation, Generated

Journal: EFORT Open Reviews

Article Title: Characterisation of 3D-printed acetabular hip implants

doi: 10.1530/EOR-23-0182

Figure Lengend Snippet: (A) A retrieved 3D-printed custom acetabular cup. (B) A retrieved 3D-printed off-the-shelf acetabular cup. (C) An SEM image of the surface of a retrieved 3D-printed implant where tissue has integrated into the porous structure. (D) An image of the surface of a retrieved implant indicating (i) strut thickness, (ii) pore size, and (iii) strut separation; features that can be compared with an unused implant.

Article Snippet: Figure 4 (A) A render of a custom 3D-printed acetabular cup created using Micro-CT data and imported into analysis software (Simpleware, Synopsys, Exeter, UK).

Techniques: Pore Size